Gene polymorphism of FTO rs8050136 and CDKAL1 rs10946398 and their association with type 2 diabetes mellitus in the Egyptian patients

Abstract Background An extensive quest for genetic variables impacting the susceptibility type 2 diabetes mellitus (T2DM) and other cardiometabolic disorders has been sparked by global growth in frequency of those widespread ailments. Although association between several SNPs T2DM reported prior research, little is known about FTO rs8050136, CDKAL1 rs10946398 Egyptian population. This study aims to investigate these two identified diabetic patients ascertain their underlying influences on T2DM. included 50 healthy subjects. Each individual underwent a clinical assessment total body examination, laboratory investigations including liver enzymes, fasting blood sugar, glycated hemoglobin (HbA1C), lipid profile. The DNA Purification Kit was used separate genomic from whole blood. Gene polymorphism detected via PCR-REFLP PCR-AS methods. Results There significant presence C allele gene at rs8050136 among studied people. patient group had considerably higher "AC" genotype "C" ( P < 0.05). Additionally, only wild-type homozygous “GG” found all cases. Conclusions "CC" significantly associated with However, no result may be attributed small sample size or rare incidence this SNP suggests verifying findings larger looking into relationship additional polymorphisms.